Testosterone, Free (Dialysis) and Total LC/MS/MS

Test Code

CPT Codes
84402, 84403

Total Testosterone, Free Testosterone (calculated)

Preferred Specimen
0.9 mL serum collected in a red-top tube (no gel)

Minimum Volume
0.38 mL

Other Acceptable Specimens
Plasma collected in: Sodium or lithium (green-top) tube


Collect blood in a no additive red-top vaccutainer. Allow blood to clot. Centrifuge and pipette serum into a plastic vial.  Do not send serum collected in a serum separator tube (SST).

Transport Container
Plastic screw-cap vial

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 2 years

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Serum Separator Tube (SST®) • Gross hemolysis • Gross lipemia

Calculation (CALC) • Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) • Tracer Equilibrium Dialysis

Reference Range
See Laboratory Report

Clinical Significance
Helpful in assessing testicular function in prepubescent hypogonadal males and in managing hirsutism, virilization in females.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.